HT-29 – Wikipedia, HT-29 ATCC ® HTB-38™ Homo sapiens colon colorectal adenocar, HT-29 ATCC ® HTB-38™ Homo sapiens colon colorectal adenocar, Though HT-29 cells can proliferate in cell culture lacking growth factors with a doubling time of around 4 days, the doubling time can be reduced to one day with added fetal bovine serum. The cells have high glucose consumption, and in standard medium containing 25 mM glucose and 10% serum, remain undifferentiated. References, The growth temperature for HT-29 is 37 . o. C ± 1 . o. C A 5 % + 1 % CO. 2 . in air atmosphere is recommended. Growth Properties Population doubling time (PDT) is approximately 23 h (Figure 4). Page . 3 . of . 26, 12/1/1989 · Human colon adenocarcinoma cell (line HT29) are able to proliferate in a defined (serum-free) medium containing no added growth factors in such conditions, their doubling time is 3 to 4 days (on serum-coated dishes) or 2 to 3 days (on an autologous extracellular matrix) compared with 1 day in the presence of fetal calf serum.
In such conditions, after a lag time (4 to 5 days) the HT29 cells are able to proliferate, although at a slower rate than in serum-containing medium ( doubling time , 3 to 4 days) (Fig. 1, circles) versus 1 day in the presence of serum (not shown). When PCS is added during the lag phase, Research. Research.
Though HT-29 cells can proliferate in cell culture lacking growth factors with a doubling time of around 4 days, the doubling time can be reduced to one day with added fetal bovine serum. The cells have high glucose consumption, and in standard medium containing 25 mM glucose and 10% serum, remain undifferentiated.
Doubling time: About 24 hrs Freeze Medium: CM-2 (CLS order number: 800225, 25ml, 800250, 50ml), serum free Freezing recovery: Slow the cells need roughly 48 hrs to settle and adhere.
HT-29 cells are negative for CD4, but there is cell surface expression of galactose ceramide (a possible alternative receptor for HIV). The line is positive for expression of c-myc, K-ras, H-ras, N-ras, Myb, sis and fos oncogenes. The p53 antigen is overproduced, and there is a G -> A mutation in codon 273 of the p53 gene resulting in an Arg …
In agreement with these observations, we have found that the growth rate of HT-29-MDR cells (mean doubling time of 96 h) is significantly slower than that of their parental drug-sensitive cells (mean doubling time of 24 h). Hence, one functional consequence of the up-regulation of caveolins in MDR cells is a slower rate of proliferation, which …
